Document 0073
 DOCN  M9490073
 TI    Comparison of biological properties of feline immunodeficiency virus
       isolates using recombinant chimeric viruses.
 DT    9411
 AU    Kohmoto M; Miyazawa T; Tomonaga K; Kawaguchi Y; Mori T; Tohya Y; Kai C;
       Mikami T; Department of Veterinary Microbiology, Faculty of
       Agriculture,; University of Tokyo, Japan.
 SO    J Gen Virol. 1994 Aug;75 ( Pt 8):1935-42. Unique Identifier : AIDSLINE
       MED/94321983
 AB    The biological properties of homogeneous populations of feline
       immunodeficiency viruses derived from infectious, molecular clones of
       the TM1, TM2 and Petaluma strains were compared. Differences in
       infectivity for Crandell feline kidney (CRFK) cells, and in syncytium
       formation and replication kinetics in a feline T lymphoblastoid cell
       line (MYA-1 cells) were observed. To investigate the basis of these
       differences between the TM2 and Petaluma strains, we first compared the
       basal promoter activity of the long terminal repeat which is a highly
       divergent region, but no significant difference in activities was found
       in CRFK cells. We then constructed two recombinant chimeric clones which
       carry gag, pol, vif, and ORF A from the heterologous virus. From
       analyses using the chimeric clones, it was revealed that efficient virus
       growth in CRFK cells and MYA-1 cells was regulated by the gag, pol, vif
       and ORF A regions, whereas viral determinants of infectivity for CRFK
       cells, and syncytium formation and cytopathogenicity in MYA-1 cells,
       were located in the env region.
 DE    Animal  Cats  Cells, Cultured  Comparative Study  Cytopathogenic Effect,
       Viral  DNA, Recombinant  Immunodeficiency Virus, Feline/*GROWTH &
       DEVELOPMENT/*GENETICS/  ISOLATION & PURIF/PATHOGENICITY  Phenotype
       Promoter Regions (Genetics)/GENETICS  Repetitive Sequences, Nucleic
       Acid/GENETICS  Support, Non-U.S. Gov't  T-Lymphocytes/*MICROBIOLOGY
       *Variation (Genetics)  Viral Proteins/GENETICS  Virulence/GENETICS
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).